Cell Culture:Article Title: NDR1 increases NOTCH1 signaling activity by impairing Fbw7 mediated NICD degradation to enhance breast cancer stem cell properties
Article Snippet: MCF-7 cells were obtained from the ATCC and were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific). .. SUM149 cell line was obtained from Asterand and was cultured in Ham's F-12 supplemented with 5% fetal bovine serum. ..
Article Title: Lipocalin 2 promotes inflammatory breast cancer tumorigenesis and skin invasion
Article Snippet: .. The SUM149 cell line was purchased from Asterand (Detroit, MI, USA), and MDA‐IBC3 cell line was generated in Dr. Woodward's laboratory [ , ] and cultured in Ham's F‐12 media supplemented with 10% FBS (GIBCO, Thermo Fisher, Carlsbad, CA, USA), 1 μg·mL −1 hydrocortisone (#H0888, Sigma‐Aldrich, St. Louis, MO, USA), 5 μg·mL −1 insulin (#12585014; Thermo Fisher), and 1% antibiotic‐antimycotic (#15240062; Thermo Fisher). .. HEK293T cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% penicillin and streptomycin (#15140122; Invitrogen, Carlsbad, CA, USA).
other:Article Title: Sulforaphene Interferes with Human Breast Cancer Cell Migration and Invasion through Inhibition of Hedgehog Signaling.
Article Snippet: Journal of Agricultural and Food Chemistry is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.
Article Title: Pharmacological targeting of GLI1 inhibits proliferation, tumor emboli formation and in vivo tumor growth of inflammatory breast cancer cells
Article Snippet: The SUM149, SUM159 and SUM190 cell lines were obtained from Asterand Inc (Detroit, MI) and cultured per manufacturer’s instructions and as described previously [35].
Article Title: Preferential Inhibition of Wnt/β-Catenin Signaling by Novel Benzimidazole Compounds in Triple-Negative Breast Cancer
Article Snippet: SUM149 and SUM159 cell lines were purchased from Asterand (Detroit, MI, USA) and cultured in DMEM/F12 with 5% FBS, hydrocortisone and insulin.
Article Title: Soluble E-cadherin Drives Brain Metastasis in Inflammatory Breast Cancer
Article Snippet: SUM149,and SUM190 cell line was purchased from Asterand (Detroit, MI), and the MDA-IBC3 cell line was generated in the laboratory of Dr. Wendy Woodward at MD Anderson., The human TNBC cell line BCX010, derived from a patient with triple-negative inflammatory breast cancer, was generously donated by Dr. Funda Meric-Bernstam (MD Anderson Cancer Center) .The cell culture conditions were described previously., HEK293T cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS and 1% penicillin and streptomycin (#15140122, Invitrogen, Carlsbad, CA, USA) at 37oC in a humidified incubator with 5% CO2.
Article Title: Cyclin E overexpression as a biomarker for combination treatment strategies in inflammatory breast cancer
Article Snippet: Cell culture conditions SUM149 and SUM185 cell lines were obtained from Asterand, KPL4 cells were obtained from Naoto Ueno (MD Anderson), and the remaining cell lines were purchased from American Type Culture Collection (ATCC).
Article Title: Photothermal ablation of inflammatory breast cancer tumor emboli using plasmonic gold nanostars
Article Snippet: SUM149 (inflammatory ductal carcinoma) and SUM190 (primary human inflammatory luminal breast carcinoma) cell lines were obtained from Asterand, Inc., and cultured as previously described. rSUM149 cells, which exhibit multidrug resistance, were synthesized in our laboratory from SUM149 cells and cultured in a manner similar to SUM149 cells with 7.5 μM GW583340 (Sigma-Aldrich, St Louis, MO, USA) added a day after splitting for each passage as described previously., BT474M1 (invasive ductal carcinoma) provided by Dr Timothy Clay at Duke University, was cultured in Dulbecco’s Modified Eagle’s Medium/F12 (Sigma-Aldrich) supplemented with 10% fetal bovine serum (Sigma-Aldrich) and 1% penicillin/streptomycin.
Multiple Displacement Amplification:Article Title: Lipocalin 2 promotes inflammatory breast cancer tumorigenesis and skin invasion
Article Snippet: .. The SUM149 cell line was purchased from Asterand (Detroit, MI, USA), and MDA‐IBC3 cell line was generated in Dr. Woodward's laboratory [ , ] and cultured in Ham's F‐12 media supplemented with 10% FBS (GIBCO, Thermo Fisher, Carlsbad, CA, USA), 1 μg·mL −1 hydrocortisone (#H0888, Sigma‐Aldrich, St. Louis, MO, USA), 5 μg·mL −1 insulin (#12585014; Thermo Fisher), and 1% antibiotic‐antimycotic (#15240062; Thermo Fisher). .. HEK293T cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% penicillin and streptomycin (#15140122; Invitrogen, Carlsbad, CA, USA).
Generated:Article Title: Lipocalin 2 promotes inflammatory breast cancer tumorigenesis and skin invasion
Article Snippet: .. The SUM149 cell line was purchased from Asterand (Detroit, MI, USA), and MDA‐IBC3 cell line was generated in Dr. Woodward's laboratory [ , ] and cultured in Ham's F‐12 media supplemented with 10% FBS (GIBCO, Thermo Fisher, Carlsbad, CA, USA), 1 μg·mL −1 hydrocortisone (#H0888, Sigma‐Aldrich, St. Louis, MO, USA), 5 μg·mL −1 insulin (#12585014; Thermo Fisher), and 1% antibiotic‐antimycotic (#15240062; Thermo Fisher). .. HEK293T cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% penicillin and streptomycin (#15140122; Invitrogen, Carlsbad, CA, USA).
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